منابع مشابه
A homologous radioimmunoassay for bullfrog basic gonadotropin.
A double antibody radioimmunoassay (RIA) for the measurement of bullfrog basic gonadotropin (GTH) is described. The antiserum was raised against a highly purified bullfrog basic GTH. Bullfrog basic GTH-IV (pI=9.33) was used for radioiodination and as a reference standard. The dissociation constant of the binding of the antibody to bullfrog basic GTH-IV was 8.28×10-12M. The standard curve was ob...
متن کامل[Purification of subunits from human chorionic gonadotropin and radioimmunoassay].
A crude hCG with an activity of about 3,000 IU per mg was purifed to 10,000--15,000 IU per mg of dry weight using Amberlite CG-50 chromatography combined with DEAE-Sephadex A-50 and Sephadex G-75. The alpha and beta subunits of hCG were prepared by urea-treatment of the hormone and isolated by DEAE-Sephadex A-50 chromatography. Further purification of the subunits was achieved by gel filtration...
متن کاملA radioimmunoassay for isoniazid.
A sensitive radioimmunoassay is described, for measuring isoniazid in therapeutic and subtherapeutic concentrations. This radioimmunoassay was used in an exploratory clinical study to measure, as a function of time, the concentration of this drug in plasma of patients receiving isoniazid therapy.
متن کاملRadioimmunoassay for human prothrombin.
I describe a radioimmunoassay for human prothrombin, with use of a double-antibody technique. Antiserum raised in rabbits was absorbed with Al(OH)3 and heated to 56 degrees C for 30 min. 125I-labeled prothrombin retaining more than 90% of its biological activity was prepared by the iodine monochloride method. The mean concentration of prothrombin in plasma of 12 normal individuals was 100 +/- 2...
متن کاملRadioimmunoassay for Cyclic Nucleotides
Sensitive and specific radioimmunoassays have been developed for cyclic adenosine 3’, 5’-monophosphate (CAMP), cyclic guanosine 3’ ,5’-monophosphate (cGMP), cyclic inosine 3’ ,5’-monophosphate (cIMP), and cyclic uridine 3’, 5’monophosphate (cUMP). These assays are based upon competition of the cyclic nucleotide with isotopically labeled cyclic nucleotide derivatives for binding sites on specifi...
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ژورنال
عنوان ژورنال: NIPPON SUISAN GAKKAISHI
سال: 1987
ISSN: 1349-998X,0021-5392
DOI: 10.2331/suisan.53.995